Several key principles for Design-based stereology ensure accuracy of the data.
Principles And Practices Of Unbiased Stereology: An Introduction For Bioscientists. For a comprehensive description of the use of Design-based stereology in neuroscience, see Schmitz and Hof 71. The design includes making sure the combination of the tissue collection (e.g., sectioning method) and the nature of and orientation of the virtual geometric shapes used to probe the tissue obviate the need for knowledge about the geometrical features being studied to make a model. Neuroscience Letters xxx (2013) xxxxxx Contents lists available at SciVerse ScienceDirect Neuroscience. Long JM, et al., What Counts In Brain Aging Design-Based Stereological Analysis Of Cell Number. The design-based methods eliminate the need for using information about the geometry of the objects to be investigated, resulting in more robust data because potential sources of systematic errors in the calculations are eliminated.ĭesign-based stereology considers solutions in which models are avoided. Older stereological methods were “ model-based”, this means that they used models based on the geometric properties of the objects being studied. The term design-based is used to describe newer methods in stereology whose probes and sampling schemes are ‘designed’, that is, defined a priori, so that the methods are independent of the size, shape, spatial orientation, and spatial distribution of the geometrical features to be investigated. West MJ. Geuna S.Appreciating the difference between design-based and model-based sampling strategies in quantitative morphology of the nervous system.Journal of Comparative Neurology 2000 427:333-9. It primarily focuses on geometrical features of objects such as number, density, length, area, and volume. Rules must be followed to achieve this independence, and if they are followed correctly than bias is eliminated. Design-based stereology in neuroscience.Neuroscience 2005 130: 813-31. Stereology is a quantitative and comparative method that utilizes planes, lines, and points for the estimation of three-dimensional parameters in morphological studies. The term “design-based” is used to describe newer methods in stereology whose probes and sampling schemes are ‘designed’, that is, defined a priori, so that the methods are independent of the size, shape, spatial orientation, and spatial distribution of the geometrical features to be investigated.
0 kommentar(er)
